| 2017/04/22 | Tonan Maebashi |  | 1-0 |  | Tokyo Int. University | | | | |
| | Gifu Kyoritsu University |  | 1-3 |  | Niigata University | | | | |
| | Blaublitz Akita |  | 1-2 |  | Kokushikan University | | | | |
| | Nagano Parceiro |  | 1-0 |  | Suzuka Unlimited FC | | | | |
| | Iwaki |  | 8-2 |  | Norbritz Hokkaido | | | | |
| | Tochigi Uva |  | 6-1 |  | Parafrente Yonezawa | | | | |
| | Nara |  | 0-1 |  | Vanraure Hachinohe | | | | |
| | Azul Claro |  | 1-0 |  | Fukui United | | | | |
| | Hokuriku University |  | 0-2 |  | Banditonce Kakogawa | | | | |
| | FC Tokushima |  | 1-2 |  | MD Nagasaki | | | | |
| | Miyazaki Sangyo-keiei University |  | 2-1 |  | Tokuyama University | | | | |
| | Arterivo Wakayama |  | 2-3 |  | Maruyasu Okazaki | | | | |
| | FC Osaka |  | 4-1 |  | Saga University | | | | |
| 2017/04/23 | Ryukyu |  | 5-5
(3-5
Pen.) |  | Imabari | | | | |
| | Nirasaki Astros |  | 1-5 |  | Briobecca Urayasu | | | | |
| | Giravanz Kitakyushu |  | 10-0 |  | Aruverio Takamatsu | | | | |
| | Verspah Oita |  | 2-1 |  | Kochi United | | | | |
| | Honda FC |  | 3-1 |  | Biwako Seikei Sport College | | | | |
| | Gainare Tottori |  | 0-1 |  | Kagoshima United | | | | |
| | YSCC |  | 1-2 |  | University of Tsukuba | | | | |
| | Kataller Toyama |  | 1-0 |  | Amitie SC Kyoto | | | | |
| | Grulla Morioka |  | 2-0 |  | Sony Sendai | | | | |
| | SRC Hiroshima |  | 1-0 |  | Mitsubishi Mizushima | | | | |
| | Kumamoto Teachers Club |  | 0-4 |  | Kagura Shimane | | | | |
18/04 - 21:30
19/04 - 13:30
19/04 - 15:45
19/04 - 16:00
19/04 - 20:00
