| 2022/05/21 | Imabari |  | 1-4 |  | Okinawa SV | | | | |
| | Matsumoto Yamaga |  | 3-2 |  | Hokuriku University | | | | |
| | Vertfee Yaita |  | 7-0 |  | Nagai | | | | |
| | Sapporo University |  | 1-0 |  | Yamanashi Gakuin University | | | | |
| | Gifu |  | 3-2 |  | Chukyo University | | | | |
| | Arterivo Wakayama |  | 2-4 |  | Kansai University | | | | |
| | Sony Sendai |  | 3-1 |  | Tokyo Int. University | | | | |
| | Honda Lock |  | 3-0 |  | Marrygold Kumamoto | | | | |
| | Fukushima United |  | 7-1 |  | North Asia University | | | | |
| 2022/05/22 | University of Tsukuba |  | 2-2 |  | Briobecca Urayasu | | | | |
| | Fukuyama City |  | 3-2 |  | FC Tokushima | | | | |
| | Kataller Toyama |  | 1-0 |  | Fujieda MYFC | | | | |
| | MIO Biwako Shiga |  | 1-2
Pro. |  | International Pacific University | | | | |
| | MD Nagasaki |  | 1-2 |  | Kagura Shimane | | | | |
| | Vanraure Hachinohe |  | 0-1 |  | Niigata University | | | | |
| | Kagoshima United |  | 3-1 |  | Giravanz Kitakyushu | | | | |
| | Jobu University |  | 0-1 |  | Fuji University | | | | |
| | Fukui United |  | 0-1 |  | Doshisha University | | | | |
| | Nara |  | 0-1
Pro. |  | Honda FC | | | | |
| | Toin University |  | 1-0 |  | Rissho University | | | | |
| | Gainare Tottori |  | 0-4 |  | Verspah Oita | | | | |
| | Saga LIXIL |  | 0-2 |  | Kochi United | | | | |
| | Suzuka Point Getters |  | 3-0 |  | Cento Cuore Harima | | | | |
| | Takamatsu High School |  | 0-3 |  | Tokuyama University | | | | |
25/04 - 23:00
25/04 - 23:00
26/04 - 15:45
